{"id":294,"date":"2026-06-16T22:18:39","date_gmt":"2026-06-16T15:18:39","guid":{"rendered":"https:\/\/dnacenter.thaizone.net\/datacolect\/"},"modified":"2026-06-16T22:18:39","modified_gmt":"2026-06-16T15:18:39","slug":"datacolect","status":"publish","type":"page","link":"https:\/\/dnacenter.thaizone.net\/en\/datacolect\/","title":{"rendered":"Sample Collection"},"content":{"rendered":"
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Sample Collection<\/h1>\n<\/div>\n
Guidance on selecting tests to order<\/strong><\/div>\n
1. Chromosome analysis from blood <\/div>\n
White blood cells (lymphocytes) are the most readily available source for chromosome analysis. The diagnosis of chromosomal abnormalities is performed by culturing cells from blood (lymphocyte culture), in which the blood sample is cultured to increase the cell count, taking approximately 1 week. <\/div>\n
Patients with various abnormalities who should be tested include: <\/div>\n
1.\t<\/span>Patients with abnormal morphogenesis<\/div>\n
2.\t<\/span>Couples who have previously had a child with abnormal characteristics, or who have a history of intrauterine fetal death, neonatal death after birth, or recurrent miscarriage (habitual abortion) of unknown cause<\/div>\n
3.\t<\/span>Female patients who have never menstruated (amenorrhea) <\/div>\n
4.\t<\/span>Children with growth or developmental problems, such as developmental delay, intellectual disability, ambiguous genitalia, multiple congenital anomalies, or individuals with intellectual disability and developmental delay accompanied by dysmorphic features, etc. <\/div>\n
 <\/div>\n
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Test limitations <\/div>\n
Diagnosis of chromosomal abnormalities of small size, such as microdeletion, microduplication, or cryptic chromosomal rearrangement (molecular cytogenetics or molecular genetics techniques may be recommended for diagnosis) <\/div>\n
Sample collection method<\/div>\n
Method 1<\/div>\n
1. Use a 5 cc sterile disposable syringe to draw up Heparin and rinse the syringe thoroughly with Heparin, then squeeze out all excess Heparin, leaving only a small amount coating the syringe. Replace with a new needle and prepare to draw the patient's blood.<\/div>\n
2. Cleanse the arm to be drawn thoroughly with soap, then wipe with Betadine, using sterile precautions as in a blood culture. If Betadine is not available, alcohol may be used, but you must wait until it dries before drawing blood.<\/div>\n
3. Draw approximately 3-5 cc of the patient's blood into the syringe, then cap the needle securely. Shake to mix the blood and Heparin together, and send the entire syringe for testing.<\/div>\n<\/div>\n
 <\/div>\n
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 <\/div>\n
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Method 2 <\/div>\n
Draw 3-5 cc of blood and collect it in a sterile tube containing an anticoagulant such as sodium heparin or lithium heparin, then send it. If it cannot be sent immediately, store it in a refrigerator at 4\u00b0C. For young children, at least 1-2 cc should be obtained.<\/div>\n
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 <\/div>\n
Every time a sample is submitted for testing, the details on the test request form must be specified as clearly and completely as possible. Once the sample has been collected, the specimen should be sent immediately by promptly notifying the Chromosome Center to send a staff member to collect it (the specimen should reach the center within 24 hours after the draw). If it cannot be sent immediately, store it at a temperature of 2-8 degrees Celsius.<\/div>\n
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